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. 2019 Nov 1;10:2510. doi: 10.3389/fmicb.2019.02510

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Copyright © 2019 Gonçalves, Ferreira, Gonçalves, Turner, Leandro, Salema-Oom, Santos and Gonçalves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolite production and mannitol dehydrogenase (Mtdh) activities in St. bombicola wild type and deletion mutants. (A) Metabolite production in St. bombicola wild type and deletion mutants (mtdh1Δ, mtdh2Δ, mtdh1Δmtdh2Δ) cultivated in YP supplemented with 10% (w/v) glucose and 10% (w/v) fructose (20FG) at 30°C for 76 h. (B) Mannitol and fructose production in St. bombicola wild type, mtdh1Δ, mtdh1Δmtdh2Δ cultivated in YP medium supplemented with 20% (w/v) glucose at 30°C for 76 h. (C) Mtdh activities in cell-free extracts of wild type St. bombicola (black dotted line) and mtdh1Δ (blue lines represent two biological replicates). The hexokinase assay served as a control for the quality of the extracts.