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. 2019 Nov 1;10:2510. doi: 10.3389/fmicb.2019.02510

FIGURE 4.

FIGURE 4

Effect of MtDH deletion on fructophily and on biomass yield at different growth temperatures. (A) Sugar consumption profiles of St. bombicola wild type, mtdh1Δ and mtdh1Δmtdh2Δ mutants. Strains were cultivated in YP supplemented with 10% (w/v) glucose and 10% (w/v) fructose (20FG) for 150 h at 30°C. Experiments were performed with two biological replicates. (B) Growth of wild type, mtdh1Δ, mtdh2Δ and mtdh1Δmtdh2Δ in YP supplemented with 10% (w/v) glucose and 10% (w/v) fructose (20FG) for 150 h at different temperatures: 25, 30, and 32.5°C. (C) Growth of St. bombicola wild type, mtdh1Δ and mtdh1Δmtdh2Δ in YP supplemented with 1% (w/v) glucose and 1% (w/v) fructose (2FG) at 30 and 32.5°C. (D,E) Growth of St. bombicola wild type, mtdh1Δ and mtdh1Δmtdh2Δ in YP supplemented with 20% (w/v) fructose (20F) (D) or 20% (w/v) glucose (20G) (E) for 150 h at 25, 30, and 32.5°C. (F) Growth of St. bombicola wild type, mtdh1Δ, mtdh1Δmtdh2Δ in (2FG) supplemented with 18% (w/v) mannitol (left) or 18% (w/v) sorbitol (right) at 32.5°C. St. bombicola mtdh1Δsor1Δ was only grown in 2FG supplemented with 18% (w/v) sorbitol. Raw data can be found in Supplementary Data S1.