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. 2019 Nov 7;9:16168. doi: 10.1038/s41598-019-52507-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the affinities of TPB and the bispecific antibodies T/P-biVLC and PHC/TLC to the FcγRIIIa (A) and HER2 oncoprotein (B) on the cell surface as shown by flow cytofluorimetry. (A) Binding of TPB, T/P-biVLC and PHC/TLC to СНО-К1.С16-cells with exposed FcγRIIIa on the surface. Herceptin is used as control. Cells were incubated with antibodies at concentrations from 0.25 to 20 µg/ml. The ordinate axis corresponds to the percentage of cells bound to antibodies as indicated by fluorescein isothyocyanate (FITC) fluorescence. The mean values with standard errors are indicated. (B) Binding of TPB, T/P-biVLC and PHC/TLC to HER2 oncoprotein on the surface of BT-474 cells. Herceptin is used as control. Cells were incubated with antibodies at concentrations from 0.1 to 20 µg/ml. The ordinate axis corresponds to the percentage of cells bound to antibodies as indicated by FITC fluorescence. The mean values with standard errors are indicated.