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. 2019 Nov 7;9:16168. doi: 10.1038/s41598-019-52507-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the disulfiram effects on the proliferation of cancer cells in vitro and the accumulation of endogenous formaldehyde in them. (A) - Inhibition of proliferation of BT-474 cells by different concentrations of disulfiram according to the results of the MTT test. Control, cells incubated with the corresponding amount of vehicle (DMSO). The mean values and standard errors are given. p < 0.001 (Mann-Whitney test) for statistical significance of the difference compared to the control. (B) – The content of intracellular endogenous formaldehyde normalized to total protein content in BT-474 cell lysates after DSF (1 μM) treatment. The mean values and standard errors are given. **p < 0.01 (Mann-Whitney test) for statistical significance of the difference compared to the control.