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. 2019 Nov 7;9:16257. doi: 10.1038/s41598-019-52723-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acetylation on K69 and K285 lysine residue promotes DVL-1 nuclear localization in MDA-MB-468 cells. (A) Stable expression of empty vector (EV), N-terminal HA-epitope tagged DVL-1 wild type (WT), HA-tagged deacetylation mutants (K to R), HA-tagged acetylation mutants (K to Q) on two lysine residues, K69 and K285 in MDA-MB-468 cells. (B) Immunofluorescence staining of empty vector (EV), N-HA-tagged DVL-1 (WT), K69R mutant (N-HA-K69R), K69Q mutant (N-HA-K69Q), K285R mutant (N-HA-K285R), K285Q mutant (N-HA-K285Q), and controls such as a mutation on DVL-1 nuclear localization signal: N-HA-tagged DVL-1-NLS mutant (N-HA-NLSm), and a mutation on DVL-1 nuclear export signal: N-HA-tagged DVL-1-NES mutant (N-HA-NESm) proteins in stably expressing MDA-MB-468 cells. Merge of N-HA-DVL-1 (red) and nuclear staining (blue) proteins is shown as HA/DAPI for each of the mutant. Merge of actin (green) and N-HA-DVL-1 (red) proteins is shown as HA/Actin for each of the mutant.