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. 2019 Nov 7;9:16253. doi: 10.1038/s41598-019-52571-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term increase in cell K⁺ content precedes DNA synthesis and accompanies transformation of small T cells into large blasts. (A) The protein content increase in PBL stimulated by PHA (10 µg/ml) for 48 hours. Data are means ± SEM of twelve experiments performed triplicate. *Significant difference from the initial value at time 0 (resting PBL) was tested by one-way ANOVA with Tukey’s post hoc tests, P < 0.05. (B) The time course of CD25 expression on small and large PBL stimulated by PHA. Data are means ± SEM of one representative experiment of three. (C) Flow cytometric analysis of cell cycle phase distribution in PHA-stimulated PBL: the percentage of cells in S and (G₂ + M) phases. (D) The time course of CD25 expression in PBL stimulated by PHA for 48 hour. Resting or PHA-stimulated PBL were stained with PE-labeled CD25 Abs and analyzed by flow cytometry. (C) and (D) – representative data of three experiments with PBL from different donors. Control - non-stimulated, resting PBL.