Table 1.

Cell K⁺, Na⁺ and water contents, and K⁺ concentration in human PBL during transit from quiescence to proliferation.

	Incubation	Cation content, µmole/g		Cell water, mL/g protein	[Ki], mM	S + G2 + M, %
		Ki	Nai
1	Resting PBL	613 ± 12	110 ± 13	4.83 ± 0,15	126	0,7 ± 0.01
2	PHA, 5 h	500 ± 24	215 ± 23	ND	ND	ND
3	PHA, 24 h	742 ± 21	140 ± 12	6.31 ± 0,37	118	1.9 ± 0.2
4	PHA 48 h	820 ± 47	173 ± 21	7.49 ± 0.41	110	38.9 ± 4.5
5	PDBu + I	760 ± 30	193 ± 25	ND	ND	41.5 ± 5.8
	Anti-CD3 + IL-2, 24 h	700 ± 50	133 ± 48	6.1 ± 0.25	115	1.3 ± 0.02
6	Anti-CD3 + IL-2, 48 h	785 ± 51	148 ± 16	7.03 ± 0.6	114	40.8 ± 3,1
7	Competent PBL (0.8PHA, 24 h)	648 ± 37	103 ± 16	ND	ND	1.3 ± 0.02
8	Competent PBL + IL-2, 48 h	790 ± 31	135 ± 17	ND	ND	31.0 ± 5.4
9	PHA + CsA, 48 h	548 ± 41	184 ± 8	ND	ND	18.1 ± 2.1
10	PHA + WHI-P131, 48 h	516 ± 29	177 ± 19	ND	ND	11.0 ± 3.9
11	Competent PBL + (IL-2 + WHI-P131) 24 h	599 ± 42	169 ± 20	ND	ND	5.4 ± 1.7
12	Jurkat T cells	804 ± 101	141 ± 32	5,84 ± 0.35	138	37,4 ± 3,2

From: Intracellular K⁺ and water content in human blood lymphocytes during transition from quiescence to proliferation.

Isolated PBL were incubated with 10 μg/ml PHA, or with phorbol ester (PDBu,10 nM) and ionomycin (I, 500 nM), or with anti-CD3 antibodies (3.5 µg/mL) and IL-2 (200 U/mL) for 24 or 48 h. Data for K_i, Na_i, cell protein and DNA cytometry are means ± SEM (p ≤ 0.05) of nine (PHA and PDBu + I), four (PHA with inhibitors), six (anti-CD3 antibodies with IL-2), five (competent PBL with IL-2 or with WHI-P131) experiments with PBL and of five experiments with Jurkat T cells. In each experiment, all the values were determined from 3 cultures of PBLs from one donor. Data for cell water per g protein, v_prot, was calculated as v_prot = (1 − ρ/ρ_dry)/[0.72(ρ − 1)], taking the density of cell dry mass as 1,38 g/mL and the ratio of protein to dry mass as 72%. Means ± SEM of three independent experiments on PBL from three donors.