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. 2019 Nov 7;10(11):844. doi: 10.1038/s41419-019-2067-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b The location of PITPNA-AS1 in HCC cells was tested by FISH and subcellular fractionation assays. c The involvement of PITPNA-AS1 in RISC was assessed by Ago2-RIP assay. d Five miRNAs that could bind with PITPNA-AS1 were predicted using starBase and DIANA tools. e, f The levels of five miRNAs were analyzed with qRT-PCR in two HCC cells after PITPNA-AS1 overexpression or silencing. g The binding sites between PITPNA-AS1 (wild-type or mutant) and miR-876-6p were exhibited. h Luciferase activity of PITPNA-AS1-WT or PITPNA-AS1-MUT was assessed in 293T or HepG2 cells transfected with miR-876-5p mimics or miR-NC. i The enrichment of PITPNA-AS1 and miR-876-5p in RISC was identified with Ago2-RIP assay. j The interaction between PITPNA-AS1 and miR-876-5p was determined by RNA pull-down assays. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 indicated statistically significant differences. n.s no significance, PITPNA-AS1 phosphatidylinositol transfer protein alpha antisense RNA 1, HCC hepatocellular carcinoma, RISC RNA-induced silencing complex, qRT-PCR quantitative real time polymerase chain reaction