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. 2019 Nov 7;10(11):844. doi: 10.1038/s41419-019-2067-2

Fig. 6. WNT5A silencing rescued the miR-876-5p suppression-induced cellular processes in PITPNA-AS1-silenced Hep3B cells.

Fig. 6

a Knockdown efficacy of WNT5A in Hep3B cells were measured by using qRT-PCR. b Cell viability was detected in Hep3B cells transfected with si-NC, si-PITPNA-AS1#1, si-WNT5A#1, si-PITPNA-AS1#1 + miR-876-5p inhibitor and si-PITPNA-AS1#1 + miR-876-5p inhibitor + si-WNT5A#1. Results were obtained using CCK-8 assay. For rescue assays, Hep3B cells were transfected with si-NC, si-PITPNA-AS1#1, si-PITPNA-AS1#1 + miR-876-5p inhibitor and si-PITPNA-AS1#1 + miR-876-5p inhibitor + si-WNT5A#1. c Cell proliferation in four groups was detected by EdU assay. d TUNEL assay was used to determine the apoptosis ability in four groups. e Cell migration was evaluated via transwell assay. f IF assay detected E-cadherin and N-cadherin levels in four groups. g The levels of WNT5A, E-cadherin, N-cadherin, MMP2, and MMP9 in different groups were examined using western blot assay. *P < 0.05, **P < 0.01 indicated statistically significant differences. PITPNA-AS1 phosphatidylinositol transfer protein alpha antisense RNA 1, HCC hepatocellular carcinoma, WNT5A Wnt family member 5A, CCK-8 cell counting kit 8, EdU 5-ethynyl-2’-deoxyuridine, TUNEL TdT-mediated DUTP nick end labeling, IF immunofluorescence, MMP2 matrix metalloprotein, MMP9 matrix metalloprotein 9