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. 2019 Nov 7;9:16259. doi: 10.1038/s41598-019-52770-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-146a inhibition increases TRAF6 mRNA and protein levels in non-CF macrophages. (a) TRAF6 mRNA was evaluated using RT-qPCR in LPS-stimulated non-CF macrophages 8 h after transfection with the miR-146a inhibitor (α-miR-146a-5p) or the control inhibitor (Ctr) (n = 6). (b) TRAF6 protein expression 16 h after transfection. TRAF6 was assessed by Western blotting, with GAPDH as the endogenous control. Densitometric quantification and one representative out of three independently performed experiments (inset) are shown. Data are means ± SEM; *p < 0.05 (paired t-tests).