Table 1.
Primer sequences used for the amplification of VfTTG1 and reference genes CYP2 and ELF1A, for the normalization of the RNA expression. Position in the genome and product amplicon sizes are indicated.
| Primer pair | 5′-3′ Nucleotide sequence | Position in faba bean genomic DNA | Lenght of PCR product |
|---|---|---|---|
| Vf_TTG1-F1/R1 | F1: TATGAATTCATTTTTAGTTCCCACCTAAC | 10 to 29 | 754 pb |
| R1: GTATCCGGTTGAGGACTCTCATAGATA | 737 to 763 | ||
| Vf_TTG1-F2/R2 | F2: GATGGGTCTGTTAGGATCTTTGATTT | 687 to 712 | 934 pb |
| R2: ATACAACTAACTTCATTGACTAAATATCTAC | 1590 to 1620 | ||
| Vf_TTG1-F3/R3 (qPCR) | F3: TGCAATGGCTTCATATGTAAGAGTG | 1282 to 1306 | 107 pb |
| R3: CAACAAGCAAATGCAACATCAATTTC | 1363 to 1388 | ||
| CYP2-F/R | F: TGCCGATGTCACTCCCAGAA | Reference genes for qPCR (Gutierrez et al. 2011) | |
| R: CAGCGAACTTGGAACCGTAGA | |||
| ELF1A-F/R | F: GTGAAGCCCGGTATGCTTGT | ||
| R: CTTGAGATCCTTGACTGCAACATT | |||