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. 2019 Nov 7;9:16239. doi: 10.1038/s41598-019-52813-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of DNA fragmentation. Slices were sampled after slicing (0 h) and after 48 or 96 h of incubation at either 20 or 80% O₂ (n = 3). TUNEL stainings were performed to assess DNA fragmentation in the airways (A) and parenchyma (B). Subsequent algorithmic analysis revealed TUNEL positive pixels (C). Values represent individual experiments performed in triplicate and are accompanied with the arithmetic mean (horizontal line) ± standard error of the mean (error bars). (*p < 0.05).