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. 2019 Nov 7;10(11):845. doi: 10.1038/s41419-019-2074-3

Fig. 5. Phosphorylation of eIF2α modulates RITA’s effect on apoptosis and colony formation.

Fig. 5

a Western blot analysis using extracts from MCF7 WT cells treated with increasing concentrations of RITA in presence or absence of 32 µM salubrinal for 4 h. b FACS based quantification of Annexin V and propidium iodide staining to detect early and late apoptosis in MCF7 WT cells treated with vehicle (DMSO) or 1 µM RITA in presence or absence of 32 µM salubrinal or 1 µM GSK2606414 for 4 h (n = 3). c, d Crystal violet staining of WT (c), TP53+/+ or TP53−/− MCF7 cells (d) after treatment with vehicle (DMSO) or 1 µM RITA in presence or absence of 32 µM salubrinal or 1 µM GSK2606414 (4 h treatment followed by a 10 day expansion before staining). The pictures represent representative images and quantification was performed on n = 3 experiments. All bars represent the mean +/− SD