Fig. 5.

A role of CHMP4B in recov-ER-phagy. a WB showing knockdown efficiency for siCHMP4B. b Same as Fig. 1b in MEF transfected with a scrambled siRNA. c Same as b in cells transfected with a siRNA silencing CHMP4B expression. d Quantification of EV delivery within EL in b, c (n = 11, 11 cells, respectively). Unpaired, two-tailed t test, **P = 0.0052. e Same as b in cells where recov-ER-phagy is recapitulated on SEC62 overexpression. f Same as c in cells where recov-ER-phagy is recapitulated on SEC62 overexpression. g Quantification of EV delivery within EL in e, f (n = 10, 10 cells, respectively). Unpaired t test, ***P = 0.0006. Data are representative of at least two independent experiments. Scale bars: 10 μm. h Same as Fig. 1a in cells transfected with a siRNA silencing CHMP4B expression. Quantification of BiP levels in WB, n = 3 independent experiments, mean ± SEM; unpaired, two-tailed t test, P = 0.4776. Molecular weight markers in WB are in kDa; n.s. not significant