Table 1.

Quantification of average diffusion and binding probabilities for lipases and respective mutants.

Lipase	Total tracks	Average Diffusion 10−10 [cm²/s]*	Binding probability
Native	4.804	3.5 ± 1.7	31.6%
Lid mutation 3 (L3)	62.642	4.7 ± 1.1	32.1%
Lid mutation 2 (L2)	44.523	3.1 ± 1.0	51.2%
Native product	12.432	1.7 ± 1.1	31.2%
Lid mutation 3 (L3) product	3.013	2.8 ± 1.3	30.8%
DOPE-ATTO655 (SPT)	2.267	3.7 ± 0.01	na**

^*Error corresponds to one standard deviation.

^**Not extractable/ensemble.