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. 2019 Nov 7;9:16274. doi: 10.1038/s41598-019-52822-1

Figure 4.

Figure 4

Synthetic RNA of the molecular CBPV clone. Plasmid DNAs were linearized with Sca I (RNA1) or Sac II (RNA2) and transcribed using SP6 polymerase. The synthetic RNA was injected into honey bee pupae to start the cycle of infection. Note that a DNA ladder is used for size estimation (left side).