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. 2019 Aug 5;56(12):5153–5166. doi: 10.1007/s13197-019-03986-1

Table 1.

TLC and HPTLC methods for determination of curcuminoids

Sample Stationary phase Mobile phase Solventa Wavelength LOD and LOQ References
TLC methods
 Turmeric powder Silica gel CHCl3:CH3COOH (80:20) CH3OH 436 nm Not reported Janben and Gole (1984)
 Turmeric rhizomes Silica gel plate 60F254 C6H14:CHCl3:CH3OH (10:10:1 v/v/v) CH3OH 254 nm 43–73 and 43–242 ng/spot (Sotanaphun et al. (2009)
 Turmeric rhizomes Silica gel plate 60 F254 CHCl3:C6H14:CH3OH (1:1:0.1 v/v/v) CH2Cl2 Image analysis LOD: 158 and LOD: 525 ng/mL Phattanawasin et al. (2009)
HPTLC methods
 Turmeric rhizomes LiChrosphere aluminium plates SI 60F254 CHCl3:CH3OH (98:2 v/v C3H6O 366 nm LOD: 40 and LOQ: 100 ng/mL Pathania et al. (2006)
 Turmeric rhizomes Nano Silica gel 60F 254 CHCl3:C4H8O2 (19:1 v/v) C2H6O 254 and 366 nm Not reported Green et al. (2008)
 Turmeric rhizomes Silica gel aluminum plate 60GF254 CHCl3:CH3OH (48:2 v/v) C6H6 425 nm LOD: 0.1 µg/spot Paramasivam et al. (2009)
 Turmeric powder Silica gel 60 F254 CH2C12:CH3OH (99:1) combination CH3OH 427 nm LOD: 49 and LOD: 148 ng/mL Gantait et al. (2011)
 Turmeric rhizomes Silica gel plate 60 F254 C7H8:C4H8O2:CH2O2 (9:6:0.4) C2H6O 200 and 700 nm Not reported Taha et al. (2015)
 Polyherbal formulation Silica gel aluminum plate 60 F254 C7H8:C4H8O2:CH2O2 (4.5:4:0.1, v/v/v) CH3OH 250 nm Not reported Baghel et al. (2017)

aUsed in sample preparation