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. 2019 Nov 7;9:16185. doi: 10.1038/s41598-019-52837-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GABPB1-AS1 negatively regulates GABPB1 through a post-translational mechanism. (A) Relative expression of GABPB1-AS1 determined by RT-qPCR in HepG2 cells transfected with pcDNA3.1 or GABPB1-AS1 overexpression vector. (B) RT-qPCR results showing GABPB1 mRNA expression in HepG2 cells transfected with pcDNA3.1 or GABPB1-AS1 overexpression vector. (C) Western blot analysis of GABPB1 in HepG2 cells transfected with pcDNA3.1 or GABPB1-AS1 overexpression vector. ACTIN was used as a loading control for RNA and total protein levels. (D) RT-qPCR confirmed the downregulation of GABPB1-AS1 using two different siRNAs compared with the negative control (NC). (E,F) RT-qPCR and western blot analysis of GABPB1 mRNA levels (E) and protein levels (F) after GABPB1-AS1 expression was reduced. (G,H) RT-qPCR (G) and western blot (H) confirmed the downregulation of GABPB1 mRNA and protein by two different siRNAs. (I) RT-qPCR analysis of GABPB1-AS1 mRNA levels after GABPB1 expression was reduced. Values are expressed as the means ± SD (n = 3). *P < 0.05, **P < 0.01.