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. 2019 Nov 7;10(11):848. doi: 10.1038/s41419-019-2085-0

Fig. 7. Knockdown of STAT3 prevents high glucose-induced pro-fibrotic responses in rat renal tubular epithelial cells.

Fig. 7

STAT3 was knockdown using siRNA (si-STAT3) in rat renal tubular epithelial cells (NRK-52E), the cells were stimulated with HG for 24 h for Western blot analysis; GAPDH as loading control, si-Ctrl = scramble sequence. Shown are representative blots indicating a, b extent of STAT3 knockdown; c, d VEGF; e, f ACE, AT1, and TGF-β1. Protein bands were quantified by densitometry, normalized to GAPDH, and reported relative to the vector control group. Data are presented as the mean ± SEM of four independent experiments; ##p < 0.01 versus Vector control, *p < 0.05, **p < 0.01, versus the HG