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. 2019 Nov 7;9:16241. doi: 10.1038/s41598-019-52335-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical expression pattern of CD3 and CD20 in the proximal part of caeca at hatching day. (A) Intraepithelial lymphocytes (arrowheads) expressed immunopostivity to CD3. (B) Lamina propria showed CD3-immunopositive T-lymphocytes (boxed areas) around the crypts of Lieberkuhn (CL). (C,D) CD20-positive B-lymphocytes were immunolocalised in the caecal tonsils (boxed area). Note, the negative control (boxed area) in A,C.