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. 2019 Oct 16;48:70–80. doi: 10.1016/j.ebiom.2019.09.027

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 2 — FOXO3a negatively regulates the stemness of OSCC. (a) Western blotting analysis of FOXO3a, stemness markers SOX2, ABCG2, CD44, BMI1 and differentiation marker IVL expression following FOXO3a overexpression in SCC15 and SCC25. β-tubulin was used as a protein loading control (b) Western blotting analysis of FOXO3a, stemness markers SOX2, ABCG2, CD44, BMI1 and differentiation marker IVL expression following shRNA knock down of FOXO3a in SCC15 and SCC25. β-tubulin was used as a protein loading control. (c) qRT-PCR analysis of FOXO3a, stemness markers SOX2, BMI1 and differentiation marker IVL expression following FOXO3a overexpression or knock down in SCC15 and SCC25. GAPDH was used as protein loading control. (d) GSEA to analyze the differential gene expression following down regulating FOXO3a in SCC15. Results are the mean ± SEM of three independent experiments and analyzed by GSEA, Student's t-test. * p < 0.05 ** p < 0.01 *** p < 0.001.