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. 2019 Oct 5;48:386–404. doi: 10.1016/j.ebiom.2019.08.040

Fig. 2.

Fig. 2

EBV-miR-BART22 activates the PI3K/AKT/GSK3β/β-catenin signaling pathway (a) Protein levels of PI3K, p-PI3K, AKT, p-AKT, c-Jun, β-catenin, GSK3β, p-GSK3β, c-Myc, E-cadherin, N-cadherin, Vimentin, Nanog, OCT4 and Sox2 were measured by western blot after miR-BART22 overexpression. Levels of related proteins were reduced or increased after BART22 inhibitor transfection or LY294002 treatment. GAPDH served as controls. (b) Immunohistochemistry analysis of E-cadherin, N-cadherin, Nanog and OCT4 expression. Original magnification, ×200, scale bar, 100 μm. (c) Western blotting analysis of the effect of miR-BART22 overexpression on GSK3β stability in NPC cells treated with cycloheximide and MG132 at different time points, GAPDH served as controls.