Fig. 5.

MYH9 promotes ubiquitin transcription via PI3K/AKT/c-Jun.

(a) Chromatin immunoprecipitation analysis (comparison of all groups vs. IgG group) (n = 3 independent experiments, one-way ANOVA) of c-Jun binding to the Ubc promoter. (b) The protein-DNA interactions between c-Jun and Ubc promoter were determined using the electrophoretic mobility shift assay. (c) Luciferase reporter assays (comparison of all groups vs. control group) (n = 3 independent experiments, one-way ANOVA) were performed to confirm c-Jun binding to the Ubc promoter.

(d) Protein levels of PI3K, p-PI3K, AKT, p-AKT, c-Jun and Ubc were measured by western blot in NPC cells treated with MYH9 plasmids or both treated with LY294002. (e-f) Chromatin immunoprecipitation analysis of c-Jun binding to Ubc promoter in HONE1-EBV⁺ and 5-8F cells treated with Ly294002. (g) QPCR analysis of Ubc mRNA levels in MYH9 over-expressed NPC cells with Ly294002 transfected (n = 3 independent experiments, Student's t-test). All data are presented as the mean ± SD. Experiments were repeated three times. *P < .05.