Fig. S4.

Related to Fig. 4, Fig. 5. MYH9 promote GSK3β ubiquitin protein degradation.

(a) Western blot analysis of GSK3β, p-GSK3β and β-catenin expression in MYH9 overexpressed NPC cells. (b) QPCR (comparison of all groups vs. control group) (n = 3 independent experiments, one-way ANOVA) analysis of GSK3β expression in MYH9 overexpressed NPC cells. (c) QPCR(n = 3 independent experiments, Student's t-test) analysis of Ubc expression in MYH9-overexpressed and their control NPC cells. (d) Bioinformatics analysis was used to predict the binding sites of c-Jun within promoter of Ubc. (e)(f) QPCR(n = 3 independent experiments, Student's t-test) and Western blot analysis of Ubc expression in c-Jun-overexpressed and their control NPC cells. (g) Co-immunoprecipitation and Western blotting analysis of the effect of ubiquitin on GSK3β expression in ubiquitin-overexpressed HONE1-EBV⁺ and the control cells. (h) Western blotting analysis the effects of ubiquitin on Ubc, GSK3β, and TRAF6 expression in ubiquitin-overexpressed NPC cells with MYH9-silenced.