Fig. 4.

Knockdown of vimentin reduces biliary senescence. [a] Measurement of cellular senescence by SA-β-gal staining in liver sections, original magn. 20×, Scale bar = 100 μm. Percentage of SA-β-gal-positive area. ^#p < .05 versus Mdr2^−/− mice. [b] Immunofluorescence for p16 (in green) co-stained with CK-19 (in red). Nuclei are stained with DAPI. original magn. 40×, Scale bar = 20 μm. [c–d] The mRNA expression of senescent markers was evaluated by qPCR in isolated cholangiocytes and hepatic stellate cells. *p < .05 versus WT mice; ^#p < .05 versus Mdr2^−/− mice. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)