HHSteCs treated with cholangiocyte supernatant lacking vimentin have decreased fibrotic reaction in vitro. The mRNA expression of fibrotic markers (Col1a1, Fn1 and TGF-β1), senescent markers (p16 and p21) and E-cadherin was evaluated by qPCR in HHSteCs treated with cholangiocyte supernatant collected from WT, Mdr2−/−, Mdr2−/− vimentin Vivo-Morpholino, and Mdr2−/− mismatched mice. *p < .05 vs. basal HHSteCs; #p < .05 vs. HHSteCs treated with cholangiocyte supernatant from Mdr2−/− mice.