Fig. 3.

p52-ZER6 regulates cell cycle progression and cell proliferation. (a) Total cell number of p52-ZER6-silenced HCT116 cells at the indicated time points. (b) Number of proliferative p52-ZER6-silenced HCT116 cells, as determined by the EdU incorporation assay. Representative images (left) and the percentage of EdU-positive cells to DAPI positive cells (right) were shown. (c) Different cell cycle phases of p52-ZER6-silenced HCT116 cells. Cells were stained with propidium iodide, and the percentages were determined by flow cytometry. Representative images are shown (left), and the average percentage of cells in each cell cycle phase from three independent experiments was calculated (right). (d) Protein expression levels of G₁ cyclins in p52-ZER6-silenced HCT116 cells. (e) Total cell number of p52-ZER6, p21-double knockdown HCT116 cells at the indicated time points. (f) Number of proliferative p52-ZER6, p21-double knockdown HCT116 cells, as determined by the EdU incorporation assay. Representative images (left) and the percentage of EdU-positive cells to DAPI positive cells (right) were shown. (g) Protein expression levels of G₁ cyclins in p52-ZER6, p21-double knockdown HCT116 cells. Cells transfected with control vector (shCon) were used as control. β-Actin was used as western blotting loading control. Quantitative data were expressed as mean ± SEM of three independent experiments. ^⁎P < .05; ^⁎⁎P < .01; NS: not significant (ANOVA).