Fig. 8.
Blue light stress increases NIR-AF signals in 9-month-old pigmented Abca4−/− mice. NIR-AF (red) and SW-AF (green) images of both groups were taken under identical conditions. In non-illuminated eyes, many melanosomes show only minimal or no NIR-AF (white arrowheads), while after blue light stress the overall number of melanosomes showing NIR-AF and the NIR-AF intensity increases (black arrowheads). In non-illuminated eyes, the majority of lipofuscin granules only show SW-AF (white arrows), while after illumination the number of lipofuscin granules showing co-localization of SW-AF and NIR-AF increases (black arrows). N = 7 animals per group. BF: bright field; NIR: near-infrared; SW: short wavelength.