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. 2019 Oct 17;48:619–629. doi: 10.1016/j.ebiom.2019.10.006

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Effect of EZH2 on the expression of degeneration-related genes in rat EPCs. (A, B) Western blot and its quantification showed the level of aggrecan and Collagen II, MMP13 and ADAMTS4 after treatment with IL-1β or Lenti-EZH2. (C) The mRNA expression of aggrecan and Collagen II, MMP13 and ADAMTS4 were measured by real-time PCR after treatment with IL-1β or Lenti-EZH2. (D, E) Western blot and its quantification showed the level of aggrecan, Collagen II, MMP13 and ADAMTS4 after treatment with IL-1β or siEZH2 or EPZ or combination. (F) The mRNA expression of aggrecan and Collagen II, MMP13 and ADAMTS4 were measured by real-time PCR after treatment with IL-1β or siEZH2 or EPZ or combination. All experiments were performed three times, and data are reported as the mean ± SD. *P < 0.05, **P < 0.01.