Fig. 5.

Sox-9 overexpression regulates the effect of EZH2 in EPCs. (A, B) Successful transfection of Lenti-Sox-9 in rat EPCs was analyzed by western blot and its quantification. (C) The expression of aggrecan and Collagen II were analyzed by western blot after treatment with IL-1β or siEZH2 or Lenti-EZH2 or Lenti-Sox-9 or combination. (D) Quantification of aggrecan and Collagen II immunoblots after treatment with IL-1β or siEZH2 or Lenti-EZH2 or Lenti-Sox-9 or combination. (E) Immunofluorescence of Collagen II was observed by fluorescence microscope (OLYMPUS). Scale bar: 50 μM. (F) Quantitation of immunofluorescence staining of Collagen II was detected by image J. All experiments were performed three times, and data are reported as the mean ± SD. **P < 0.01.