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. 2019 Oct 6;48:526–538. doi: 10.1016/j.ebiom.2019.09.010

Fig. 2.

Fig. 2

Induction of MX1 by miR-1248 in phSG cells. (a) Transfection of miR-1248 in phSG cells induces MX1 protein expression under both high and low calcium conditions. (b) The induction of MX1and OAS1 due to miR-1248 were dose-dependent (β-actin was used as loading control in both a and b). (c) Total RNAs from phSG cells transfected either with miR-N.C. (control) or miR-1248 were subjected to RT-qPCR. Data are shown as a mean ± S.E. from three independent experiments and presented as a relative quantification to each respective miR-N.C. control sample. The error bars represent a 95% Confidence Interval. (d) Indicated luciferase reporter plasmid was transfected into phSG cells 24 h prior to the transfection of indicate miRNAs for 48 h. Cellular lysates were used for both firefly and renilla luciferase activity. Data are shown as mean ± S.E. of RLU from three independent experiments, after normalized with renilla luciferase activity.