Fig. 3.

Secretion of IFN-β in miR-1248 transfected phSG cells. (a) Western blot analysis showed reduced miR-1248-induced MX1 expression after knockdown of IFNAR1/R2 receptors in phSG cells. (b) Quantification of secreted interferons by ELISA showed a dose-dependent increase of IFN-β. Error bars represent standard error from three independent experiments with duplicated samples. (c) Conditioned media collected after phSG cells were transfected with miR-N.C. (medium A) or miR-1248 mimic (medium B) for 24 h was incubated with phSG cells previously transfected with indicated siRNAs or miR-1248 inhibitor for 24 h before exposure to the conditioned media for another 24 h. MX1 transcript level was shown to be induced by Medium B only. Data are shown as RQ (relative quantification) over the phSG cells transfected with miR-N.C. The error bars represent a 95% Confidence Interval. RQ: relative quantification.