Fig. 7.

PPP1CA is essential for USP11-mediated promotion of CRC. (a) The effects of PPP1CA on USP11-induced cell proliferation were investigated using CCK-8 assays. SCH772984 was added as an ERK1/2 inhibitor. (b) Transwell assays were used to evaluate the migration ability in response to changes in USP11 and PPP1CA. SCH772984 was also used as an ERK1/2 inhibitor; scale bar 200um. (c, d) Effect of PPP1CA overexpression or knockdown on the growth rate of subcutaneous tumor caused by USP11 knockdown or overexpression in vivo (n = 5). (e) An in vivo liver metastatic assay was used to clarify the function of PPP1CA during the USP11-mediated promotion of CRC metastasis. *P < 0·05, **P < 0·01. P values were calculated with one-way ANOVA in Fig. a-b and Student's t-test in other figures.