Fig. 7.

STEAP4 decreases the pFAK/FAK ratio and influences EMT.

a. Co-IP using anti-Flag antibody or IgG in wild-type BC cells or cells stably expressing STEAP4–3 × Flag. Proteins immunoprecipitated were detected by western blotting with anti-FAK and anti-Flag antibodies. b. and c. Western blotting showed FAK and its phosphorylation form in T24 and UM-UC-3 cells when silencing or overexpressing STEAP4. d. FAK Inhibitor 14 could eliminate the pro-metastasis effect of STEAP4 silencing, indicated by migration and Matrigel invasion assays. Scale bar, 100 μm. e. Western blotting illustrated EMT markers expression in BC cells transfected with STEAP4 siRNAs. f. Model we propose that circPICALM sponges miR-1265 to influence the metastasis of BC. STEAP4 is a direct miR-1265 target and exerts its function on EMT through binding and inhibiting the phosphorylation of FAK. (Data are presented as the mean ± SD, n = 3. Unpaired, two-tailed student's t-test, *P<0.05, **P<0.01).