Supplementary Fig. 2.

a. Invasive abilities of BC cells overexpressing circPICALM, measured by wound healing assays. Scale bar, 100 μm. b. Colocalization of miR-1265 and circPICALM in T24 cells, demonstrated by FISH. Probe for miR-1265 was labelled by FAM and probe for circPICALM was labelled by cy3. Scale bar, 25 μm. c. and d. Relative expression of miR-1265 in BC cells transfected with mimics or inhibitor. e. and f. Migratory and Invasive abilities of BC cells treated with miR-1265 inhibitor, measured by migration, invasion, and wound healing assays. Scale bar, 100 μm. (Data are presented as the mean ± SD, n = 3. Unpaired, two-tailed student's t-test, **P<0.01).