Supplementary Fig. 3.

a. The wound healing assay showed that overexpression of circPICALM could eliminate the pro-metastasis function of miR-1265 in BC cells. Scale bar, 100 μm. b. Relative expression of STEAP4 mRNA in 40 paired primary BC (T) and adjacent normal (N) tissues, measured by RT-PCR. c. The correlation between circPICALM and STEAP4 expression in 40 BCE samples. d. and e. STEAP4 overexpression (OE) inhibited the metastasis of BC, detected by migration, invasion, and wound healing assays. Scale bar, 100 μm. (Data are presented as the mean ± SD, n = 3. a, d, and e. unpaired, two-tailed student's t-test; b, paired, two-tailed, Wilcoxon rank-sum test; c. Spearman's rank correlation. **P<0.01).