Fig. 3.

Silencing of circHIPK3 induces autophagy and sensitises CRC cells to oxaliplatin. (a) Transfection of mRFP-GFP-LC3B lentiviral vector into HCT116oxR cell line. Red puncta represent autolysosomes, and yellow puncta represent autophagosomes as visualised by confocal microscopy. Scare bar = 20 μm (b) Quantification of autophagic flux. Puncta were counted in 100 cells; ^⁎⁎⁎P < 0•001 [student's t-test]. (c) Western blot analysis to determine LC3B- II/I ratio, p62 and, beclin1 expression in HCT116oxR cells.