Fig. 2.

Histological analysis of kidney injury in DKD patients. (A) Representative images of HE, PAS, Masson and PSR staining (magnification, 100×). (B) Quantification of PAS, Masson and PSR staining. (C) Representative immunohistochemical staining (IHC) for FN and Col I (magnification, 100×). (D) Quantification of IHC staining for FN and Col I. (E) Representative western blot of FN and Col I. (F-G) Densitometry of FN and Col I was analyzed. (H–I) The mRNA levels of FN and Col I were assessed by RT-PCR. The results are expressed as the mean ± SEM, n = 5 kidneys per group, *P < .05, P values were determined by unpaired t-test.