Fig. 5.

Diabetic kidney-specific Pax2-3aFoxO1 overexpression mice ameliorated the expression of FN and Col I. (A-B) Representative images for FN and Col I production assessed by IHC analyses in mice. Original magnification, 200×. (C-D) Quantification of IHC staining for FN and Col I in mice. (E-F) The mRNA levels of FN and Col I were assessed by RT-PCR. (G) Representative western blot of FN and Col I. (H-I) Quantitative analysis of the densitometry of FN and Col I . All experiments were performed in triplicate. Data were shown as the mean ± SEM, n = 5, *P < .05 vs. the WT mice; #P < .05 vs. the WT-DM mice. P values were determined by one-way ANOVA analysis.