Supplemental Fig. 1.

The expression of FoxO1 in kidney-specific Pax2-3aFoxO1 overexpression mice. (A) Schematic of Pax2-3aFoxO1 transgene construct in which 3aFoxO1 cDNA was inserted into a vector that contained the mouse Pax2 promoter. (B) The Pax2-3aFoxO1 mice were identified by PCR amplification of the 3aFOXO1-GFP sequence (lanes 1–10, Pax2-3aFoxO1 mice; lane 11, WT mice). The length of PCR product is 490 bp. (C-D) Western blot and Immunohistochemical staining of FoxO1 expression was confirmed in Pax2-3aFoxO1 and WT mice kidneys. Note: magnification: x 400. (E) The result of sequences in Pax2-3aFoxO1 mice. *P < .05 vs. WT mice, P values were determined by unpaired t-test.