Fig. 11.

SWELL1 is required for the function of VRAC in HCC cells. (a) Morphological changes observed in SMMC-7721 and Huh7 cells after exposure to the hypotonic solution. (b) Results of the re-incubation of HCC cells after exposure to hypotonic solutions for 0, 5, or 10 min. Results were assessed by two methods: the number of the surviving cells after the exposure was counted with trypan blue staining and the representative images captured under a microscope. **P < .01. Data are represented as the mean ± SD of three independent experiments and compared using one-way ANOVA followed by a Dunnett t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)