Fig. 2.

LBH overexpression in glioma conditioned media (GCM) can promote the proliferation, invasion and angiogenesis of hBMECs

a, b: Gene set enrichment analysis (GSEA) indicates that high expression of LBH is associated with the VEGF signalling pathway and hypoxia in both the TCGA (a) and CGGA (b) databases.

c, d: Vascular endothelial cell viability increased after treatment with LBH-overexpressed U118 (c) and GSC2A (d) conditioned media, as measured by the MTS assay. (U118: p = 0.0019, GSC2A: p = 0.0053, One-Way ANOVA).

e, f: The proliferation of vascular endothelial cells increased following treatment with LBH-overexpressed U118 and GSC2A conditioned media, as measured by the EDU incorporation assay, scale bar = 100 μm. (U118: p <0.0001, GSC2A: p <0.0001, Student's t-test)

g: Representative transwell assay showing the increase in invasion of vascular endothelial cells after treatment with LBH-overexpressed U118 and GSC2A conditioned media. Scale bar = 100 μm. (U118: p = 0.0087, GSC2A: p = 0.0093, Student's t-test)

h: Representative tube formation assay showing the increase in tubulogenesis of vascular endothelial cells after treatment with LBH-overexpressed U118 and GSC2A conditioned media. Scale bar = 100 μm. (number of branches: U118: p = 0.0071, GSC2A: KD1, p = 0.0089; tubule length: U118, p = 0.0025, GSC2A: p = 0.0039, Student's t-test)

All data are shown as the mean ± SD (three independent experiments). *P < 0.05; **P < 0.01; ***P < 0.001.