Fig. 6.

ISX characterization of plasma-derived PpIX-positive EVs from control and glioma-bearing mice dosed with 5-ALA. (a) Overview of the experiment (b) Line plot showing pre-5-ALA and post-5-ALA PpIX-positive EV levels for the control (gray) and glioma (red) mice. The dotted line represents the threshold (t) of background fluorescent signal prior to 5-ALA dosing based on this population. (c) ISX scatter plot of representative control mouse plasma before 5-ALA (left) and 3 h post- 5-ALA (right). (d) ISX scatter plot of representative glioma-bearing mouse plasma before 5-ALA (left) and 3 h post-5-ALA (right). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)