Figure 5.

ADAMTS9-AS2 Modulates Transcription Factor SCRG1 through Regulating miR-942-5p

(A) Expression of mRNAs measured by qPCR after hMSCs were treated with mimic-942-5p. (B) Schematic of the miR-942-5p putative target site on ADAMTS9-AS2, Sox9, and Scrg1. (C) The luciferase reporter assay for the wide-type Sox9 and Scrg1 in the presence of miR-942-5p and the mutant Scrg1 in the presence of miR-942-5p. The values are shown as Renilla luciferase activity to firefly luciferase activity. (D and E) qPCR for mRNA levels of Scrg1 in hMSCs after downregulating and overexpressing ADAMTS9-AS2. (F and G) Immunohistochemistry assay for sections of day 14 micromass for Scrg1 protein abundance in hMSCs after downregulating and overexpressing ADAMTS9-AS2. Scale bar, 50 μm. (H and I) Quantitative statistics for dyeing results of (F) and (G) after downregulating and overexpressing ADAMTS9-AS2. Experiments were performed in triplicate and error bars represent SD of a triplicate set of experiments. Data are shown as mean ± SD; *p, 0.05, **p, 0.01; ns, non-significant.