. 2019 Oct 14;9:e00103. doi: 10.1016/j.mec.2019.e00103

Table 2.

Metabolic performance parameters of recombinant P. pastoris in glucose-limited chemostats under different μ and DO conditions.^a.

Condition^b	μ (h⁻¹)	q_S (mmol g_DCW⁻¹ h⁻¹)	q_CO2 (mmol g_DCW⁻¹ h⁻¹)	q_O2 (mmol g_DCW⁻¹ h⁻¹)	q_P (mg g_DCW⁻¹ h⁻¹)	Y_S,X (g_DCW g⁻¹)	Y_S,P (mg g⁻¹)
1 (L)	0.072 ± 0.003	0.79 ± 0.038	1.84 ± 0.101	1.84 ± 0.097	0.05 ± 0.005	0.51 ± 0.002	0.27 ± 0.042
2 (L)	0.119 ± 0.006	1.25 ± 0.031	2.77 ± 0.017	2.79 ± 0.317	0.04 ± 0.003	0.53 ± 0.011	0.16 ± 0.016
3 (M)	0.048 ± 0.002	0.48 ± 0.029	1.03 ± 0.051	1.05 ± 0.068	0.03 ± 0.001	0.55 ± 0.008	0.40 ± 0.019
4 (M)	0.101 ± 0.001	0.99 ± 0.009	1.98 ± 0.016	1.97 ± 0.076	0.03 ± 0.001	0.57 ± 0.002	0.19 ± 0.001
5 (M)	0.150 ± 0.002	1.50 ± 0.021	3.02 ± 0.052	2.96 ± 0.099	0.035 ± 0.001	0.57 ± 0.002	0.13 ± 0.002
6 (E)	0.072 ± 0.001	0.68 ± 0.007	1.21 ± 0.004	1.30 ± 0.005	0.03 ± 0.001	0.59 ± 0.004	0.26 ± 0.007
7 (E)	0.120 ± 0.001	1.14 ± 0.014	2.18 ± 0.147	2.31 ± 0.129	0.03 ± 0.001	0.58 ± 0.004	0.18 ± 0.009

Results represent the average and standard error of two or three independent experiments (biological replicates) for the extreme and the central points, respectively. In each case, the carbon balance closed satisfactorily and reconciled no less than 94.7% of the consumed carbon source (see Supplementary Table S5).

The experimental conditions are abbreviated L for low oxygenation (4% dissolved oxygen saturation), M for medium oxygenation (30% dissolved oxygen saturation), and E for excess oxygenation (56% dissolved oxygen saturation).