Figure 1.
Characterization of 2-DPAN
(A) Synthetic route of 2-DPAN. See also Figure S1.
(B) Photoluminescence spectra of 2-DAPN in tetrahydrofuran (THF)/water mixtures with different water ratios. ([2-DPAN] = 10 μM. Excitation wavelength at 405 nm).
(C) Plots of I/I0 versus water fractions of 2-DPAN, where I0 is the emission intensity in THF.
(D) The absorption spectra and photoluminescence spectra of 2-DAPN with Stokes shift of 139 nm.
(E) Photostability of 2-DPAN and Nile Red (commercial LDs tracker) under exposure to 405 and 565 nm laser with increasing number of scans (power intensity of 99%), respectively. Cells co-stained with 2-DPAN and Nile Red.
(F) Bright field.
(G) Red fluorescence collecting channel for Nile Red.
(H) Green fluorescence collecting channel for 2-DPAN.
(I) Merge imaging, the SH-SY5Y cells were pre-induced by OA for 6 h, where the concentration of OA was 1 mM.)