Figure 3.

Reduction of Fmr1 mRNA Levels Normalizes Dendritic Spine Morphology in KI Cortical Neurons

(A) Representative high-resolution confocal images showing dendritic spine morphology were assessed using NeuronStudio software and compared with the measurement obtained from control transduced neurons. Scale bars: 2 μm. All histograms present mean ± SEM values. Statistical significance was assessed by one-way ANOVA with Tukey’s multiple comparisons test. (B–G) Histograms showing the mean ± SEM values of (B) protrusion frequency in the various cultures, *p < 0.05; (C) spine length, ***p < 0.001; (D) spine head size, ***p < 0.001; (E) percentage of thin spines; (F) percentage of stubby spines; and (G) percentage of mushroom spines.