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. 2019 Nov 7;8(45):e00388-19. doi: 10.1128/MRA.00388-19

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Copyright © 2019 Pakala et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — We combined the 17 newly acquired complete EV-D68 genome sequences collected in 2018 in Kansas City with EV-D68 genome sequences available in GenBank (as of 31 January 2019) and generated a data set with a total of 628 complete genome sequences of EV-D68. Sequences were aligned using the MUSCLE program in MEGA6, with manual adjustments (15). The global phylogenetic tree of EV-D68 was inferred using the neighbor-joining method conducted in MEGA6, with the Kimura 2-parameter test and bootstrap test of 1,000 replicates (16). The tree is midpoint rooted and drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Well-supported nodes by bootstrap values over 70% are shown next to the branches. Six major clades/subclades (B1, B2, B3, C, A1, and A2) are described in the tree. New 2018 sequences sampled from Kansas City are marked in the tree as well.