Figure 1.

Characterization of Wing Imaginal Disc Morphology

(A) (i–v) The morphology changes between 48 and 96 h AEL. Maximum projection images, top and cross-section from DV axis midline views. Arrowheads point to HN, HH, HP, and LF in red, green, blue, and magenta, respectively. Scale bars are 50 μm. Due to the projection, basal folds are visible on the top view, example marked by black star on (v). (vi and vii) Lateral cross-sections along lines marked with white stars on (v).

(B) Schematic of the wing disc structure. (i) Domains are labeled, the thin peripodial layer is hardly visible on the experimental images. (ii) Top and cross-section with developmental axes and fold names labeled.

(C) (i) Wing disc size during fold formation, developmental age progresses from black to white, see STAR Methods for n. At 48 h AEL, the mean AP and DV lengths are 56 and 84 μm, respectively. Prior to 80 h AEL, 114 and 185 μm; at 88 h AEL, 128 and 222 μm. At 96 h AEL, 214 and 294 μm, the apical contour length on the DV axis is 402 μm. (ii) Fold positions normalized to DV length, error bars represent one standard deviation. Up to 88 h AEL, the positions are 0.48, 0.58, and 0.66 for NH, HH, and HP folds respectively. At 96 h AEL, they are 0.43, 0.52, and 0.61. 72–88 h AEL, NH fold n = 22, HH fold n = 29, HP fold n = 18. 96 h AEL, n = 14 for all folds. Boxes represent 25^th and 75^th percentiles, medians in red, whiskers extend the most extreme data points that are not outliers, outliers plotted with red plus.