REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Wingless MIgG1	DSHB	4D4; RRID: AB_528512
Donkey anti-mouse RRX	Jackson Immuno Research	715-295-151; RRID: AB_2340832
Chemicals, Peptides, and Recombinant Proteins
DAPI	Sigma-Aldrich	D8417; RRID: AB_2307445
Phalloidin Alexa-647	Life Technologies	A22287
Phalloidin Alexa-555	Life Technologies	A34055
Hoechst	Sigma-Aldrich	B2261
Fluoromount G Slide mounting medium	Southern Biotech	0100-01
Bovine Serum Albumin	Sigma	A7030
Triton X-100	Sigma	T8787
16% w/v formaldehyde (Prediluted with PBS to 4% for fixation protocols)	TAAB Laboratories	F017/3
EM grade formaldehyde 36%	TAAB Laboratories Equipment Ltd	F003
EM grade glutaraldehyde 25%	TAAB Laboratories Equipment Ltd	G011
Osmium Tetroxide 2%	TAAB Laboratories Equipment Ltd	O005
Potassium ferricyanide	TAAB Laboratories Equipment Ltd	P018
Dodecenyl Succinic Anhydride	TAAB Laboratories Equipment Ltd	D027
2,4,6- Tri(Dimethylaminomethyl)	TAAB Laboratories Equipment Ltd	D032
Methyl Nadic Anhydride	TAAB Laboratories Equipment Ltd	M011
TAAB 812 resin	TAAB Laboratories Equipment Ltd	T023
Tannic Acid	TAAB Laboratories Equipment Ltd	T046
Lead Nitrate	TAAB Laboratories Equipment Ltd	L005
Potassium Ferricyanide	Sigma-Aldrich	P8131
Sodium citrate	Sigma-Aldrich	S4641
Experimental Models: Organisms/Strains
w;; actin-FRT-CD2-FRT-Gal4, UAS-GFPNLS	Neufeld et al., 1998	N/A
hsFLP	Bloomington Drosophila Stock Centre	BDSC8862
Yw	Bloomington Drosophila Stock Centre
winglessspd-fg	Bloomington Drosophila Stock Centre	BDSC1005
ywhsFLP; wgspd-fg; S-T	Y.Mao	N/A
hsFLP; UAS-InRA1325D/CyO; MKRS/TM6B	N. Tapon	N/A
w; actin-FRT-y-FRT-Gal4-UAS-CD8-mCherry/CyO; arm-GFP/TM6	Y.Mao	N/A
Software and Algorithms
Microsoft Excel 16	Microsoft	N/A
GraphPad Prism 7	GraphPad Software	N/A
Matlab 2016	Mathworks	N/A
ImageJ 1.51w	(Schneider et al., 2012) https://imagej.nih.gov/ij/docs/install/osx.html	N/A
Triangle - A two-dimensional quality mesh generator and Delaunay triangulator	(Shewchuk, 2005) https://www.cs.cmu.edu/∼quake/triangle.html	N/A
Tissue morphogenesis FE simulation software	Bespoke model code developed in C++ for this study.	N/A